温州龙湾国际机场入境旅客携带植物及其产品疫情 分析及应对措施

本文通过对2013—2018年温州空港口岸截获的入境旅客植物及其产品情况进行分析,发现温州空港口岸有害生物检出种类呈增长态势。6年共查获管制的植物和植物产品7495批次,截获有害生物110种,其中检疫性有害生物13种,占比11.82%。有害生物种类以昆虫居多,共55种,占比50%;热带水果检出有害生物最多,8种热带水果中检出12种检疫性有害生物。旅客携带物的有害生物检出具有时间季节效应,疫情来源地以东南亚中国台湾、中国香港、越南和泰国等国家和地区为主等特点。根据截获温州空港口岸入境旅客携带植物及其产品检出有害生物特点,因地制宜对入境旅客携带物检验检疫提出相关应对策略。     

家蚕Ras3基因全长cDNA克隆及在感染BmNPV家蚕组织中的表达谱分析

Ras(rat sarcoma viral oncogene homolog)蛋白是GTPase超家族成员之一,该蛋白质可以将细胞外信号传导到不同的细胞,从而调控细胞的吞噬作用、凋亡、抗微生物侵染的酶防御系统等生理过程.运用RACE技术,从家蚕中肠中克隆出一个新的BmRas3基因,该基因cDNA全长987 bp,包含5'UTR(153 bp)、3'UTR(279 bp)和一个编码184个氨基酸残基的ORF(555 bp).BmRas3具有小G蛋白的典型特征,其氨基酸序列中含有3个GTP/GDP结合结构域,1个效应子结合结构域以及1个烯丙基化的CAAX基序.多重比对发现BmRas3与海波斯莫科马属蛾(Hyposmocoma kahamanoa)Rap1、斜纹夜蛾(Spodoptera litura)Rap-1b、烟草天蛾(Manduca sexta)Rap1的序列相似性较高,系统进化分析也显示BmRas3与这些蛋白质的亲缘关系最近.BmRas3在食桑期的表达量高于眠期;在感染BmNPV的家蚕中肠和脂肪体中,BmRas3的表达量有不同程度提高,且与正常对照相比差异显著,暗示BmRas3可能作为免疫活化因子,在家蚕抵抗BmNPV的入侵过程中发挥着重要作用.研究结果为下一步探讨BmRas3对BmNPV感染的应答提供了理论基础.     

库姆塔格沙漠东南部胡杨液流变化及其对环境因子的响应

于2018年4月?10月(春季4月、夏季7月、秋季9月)利用PS-TDP8树木茎流监测系统对库姆塔格沙漠东南部胡杨(Populus euphratica)的液流速率变化进行监测,采用相关分析及逐步回归法分析气象因子(太阳总辐射、空气温度、风速、空气相对湿度)对胡杨液流的影响。结果表明:胡杨茎干液流日变化趋势呈几字“宽峰型”;夏季液流的启动时间最早,液流启动在06:00,秋季液流启动时间最晚,不同季节夜间存在较高液流(10.994~26.035 cm·h^?1);树干液流速率与太阳总辐射、空气温度、风速、饱和水气压差呈正相关关系,与空气湿度呈负相关关系;影响春季、秋季胡杨液流速率的主要气象因子是太阳总辐射和空气温度,夏季为太阳总辐射和空气相对湿度;不同季节胡杨液流速率与空气温度、空气相对湿度、风速、饱和水气压差的日变化响应呈顺时针环状,与太阳辐射响应呈逆时针环状;不同季节胡杨液流速率对气象因子的响应规律存在差异。这表明胡杨为了保持水分平衡应对水分亏缺的策略不同,在一定条件下(极端干旱)通过气孔调节降低水分损失。研究结果对于明确干旱沙漠环境中胡杨水分传输对环境因子的响应规律及更深入地认识胡杨适应干旱环境的水分利用策略具有重要意义。     

Administration of probiotic Bacillus licheniformis induces growth,immune and antioxidant enzyme activities,gut microbiota assembly and resistance to Vibrio parahaemolyticus in Litopenaeus vannamei

This study aimed to elucidate the effects of dietary Bacillus licheniformis ATCC 11946 (BL) on the growth, immune and antioxidant activities, intestinal morphology and microbiota, and susceptibility to Vibrio parahaemolyticus (VP_AHPND) in juvenile pacific white shrimp, Litopenaeus vannamei (LV). Juvenile LV (initial weight = 0.63 ± 0.001) were fed diets containing varying BL concentrations (0 (BL0), 10⁶ (BL1), 10⁷ (BL2) and 10⁸ (BL3) CFU/g feed) for 8 weeks. Growth performance, immune and antioxidant enzyme activities, and intestinal morphology significantly improved in the probiotic‐treated groups than the untreated. Regardless of the treatment group, the two dominant phyla were Proteobacteria and Bacteroidetes, whereas the two dominant genera were Ruegeria and Vibrio. Increasing inclusion of probiotics in diets led to significant increase in beneficial bacterial genera (Ruegeria, and Pseudoalteromonas) and a significant decrease in some known opportunistic pathogens (Vibrio, Tenacibaculum, Photobacterium, Kangiella and Spongiimonas) with the BL3 group witnessing the best. A 7‐day challenge study with VP_AHPND showed significantly high protection in the probiotic‐treated groups, with the BL3, BL2 and BL1 obtaining 51%, 34% and 22% relative percentage survival, respectively. In conclusion, BL at 10⁸ CFU/g feed should be used to help in shrimp production since it attained the most significant improvement.     

Technical efficiency of different farm sizes for tilapia farming in China

China is the world's largest tilapia producer. This study uses the stochastic frontier function in the Cobb–Douglas model to evaluate a production frontier for tilapia aquaculture and analyses the relationship between farm size and production efficiency in China. A random sample of 300 tilapia farmers was surveyed in the main tilapia‐producing areas to study their economic efficiencies. The percentage distribution of tilapia farm technical efficiency was determined, with an average efficiency of 79%, which showed that the sample tilapia farmers operate at 21% below the production frontier and hence that they still have a chance to achieve targeted yields. The technical efficiencies of two categories (≤1 ha and >1 ha) were 78.82% and 79.27% respectively. A second‐stage analysis investigated the relationship between farm size and technical efficiency and showed a positive relationship between farm size and production efficiency based on the ordinary least‐squares model. The optimal farm size was found to be 7.50 ha. This study proposes strategies such as enlarging the farming scale moderately, thereby achieving the advantage of economies of scale, and enhancing the competitiveness of the tilapia industry in China.     

金寨毛竹纤维形态及化学成分

通过离析法制取毛竹纤维,观察并记录纤维长度和宽度,计算纤维长宽比,按照国家标准分别测定化学成分质量分数,以此对3~5年生金寨毛竹不同竹干高度、径向的纤维形态和化学成分进行研究。结果表明:3~5年生金寨毛竹平均纤维长度均大于1500μm,且随着竹龄的增加而增加,而在高度上变异规律不明显;平均纤维长宽比均大于100,在竹干高度为5.5 m处达到最大值;灰分质量分数随竹龄的增加而减小,随竹干高度的增加而增加;综纤维素质量分数随竹龄的增加而增加,随竹干高度的增加趋于稳定;苯醇抽提物和纤维素质量分数随竹龄增加先减小再增加。综上可知,金寨毛竹是优良的造纸原料。     

基于联盟链的电力物资全寿命周期管理体系研究

受限于国家电网现有的物资管理方式以及内外部数据网络连通等问题,电力物资尚未实现全寿命周期的可追溯管理。区块链技术的不断革新,为解决电力物资全寿命周期管理过程中存在的问题,提供了新的思路。文章希望通过构建电力物资全寿命周期管理体系,创新电力物资管理方式,同时通过融合联盟链技术,解决当前物资管理的数据连通模式存在的短板,为电力物资管理探索新的方向。